Análisis del polimorfismo de 21 genotipos de musa spp, mediante el uso de marcadores microsatélites

Abstract

This study was conducted in 21 accessions of different genotypes of Musa spp., from the Worldwide Collection (INIBAP Transit Center, Catholic University of Leuven, Belgium) with the objective to determine the relationship between genetic diversity and the level of resistance to the fungus Mycosphaerella fijiensis causing black Sigatoka infection. We used a total of 20 microsatellite markers, 10 derived from the genome of M. acuminata and 10 derived from the genome of M. balbisiana. Nineteen markers produced well defined bands and generated a total of 85 alleles, with an average of 5.1 alleles per primer. The average genetic diversity was 0,719 and fixation index FST = 0,409. These results show a very high genetic differentiation among the 21 genotypes of Musa spp. The hierarchical cluster analysis showed that susceptible genotypes are genetically closer to tolerant genotypes and hyper resistant genotypes are genetically more distant that tolerant and susceptible genotypes.