Evaluación del cultivo de Pseudoalteromonas luteoviolacea sobre placas porosas en biorreactor

Abstract

Cancer contributes to one of the leading causes of mortality worldwide, and conventional treatments are often associated with intense side effects and high costs that limit their accessibility. Violacein, a natural pigment with anticancer potential, faces obstacles due to its low production, which restricts its application for the development of oncological drugs. The objective of the present study is to increase violacein yield through the cultivation of Pseudoalteromonas luteoviolacea on porous plates using an optimized culture medium. Five different culture media and four porous materials (tagua, pumice stone, ceramic rings, and chicken bones) were evaluated over 240 hours, with an incubation temperature of 30°C. The results showed that UV-irradiated albumin was the most effective medium (1.35 mg/mL), while chicken bones constituted the optimal porous material, reaching values of 8.7 mg/mL. Additionally, scaling up to 1.6 L bioreactors was performed, where concentrations of 22.4 mg/mL were achieved. In Aspen Plus, chicken bones were considered as 100% carbon Raschig rings and as a catalyst. Tryptophan was considered with 100% purity, and seawater was treated as sodium chloride and water, obtaining a biomass concentration of 2 g/L in 40 hours, which subsequently remained constant. It is concluded that the combination of UV-irradiated albumin with porous chicken bone substrates optimizes bacterial growth, offering potential for biotechnological and pharmaceutical applications. Keywords: Biofilms, porous materials, Pseudoalteromonas luteoviolacea, violacein