Desarrollo y validación de un método de análisis por UPLC-DAD para la determinación de ERITROSINA (E127) en bebidas de consumo humano

Abstract

The present study aimed to determine the conformity of a UPLC–DAD method for the quantification of erythrosine (E127) in carbonated and rehydration beverages with the validation requirements established in ISO/IEC 17025:2017. For this purpose, a chromatographic procedure was developed for the identification and quantification of the analyte, using calibration curves in the range of 0.05–2.50 μg/mL and a controlled experimental design. Method validation was performed following international guidelines (Eurachem and ISO/IEC 17025:2017) and included selectivity, linearity, limits of detection and quantification, precision (repeatability and intermediate precision), accuracy, robustness, and measurement uncertainty. The method showed consistent linear behavior (R² between 0.9970 and 0.9995), absence of relevant interferences at the analyte retention time, and adequate precision and accuracy for routine use. The LOD/LOQ values were 0.0193/0.0642 μg/mL in rehydration beverages and 0.0117/0.0391 μg/mL in carbonated beverages, with a relative expanded uncertainty of 7.97% (k = 2). Finally, the method was applied to the analysis of 12 beverages from the Ecuadorian market, yielding non-detected results (<LOD), which confirms its suitability for analytical control. Keywords: Erythrosine (E127), UPLC–DAD, analytical validation, ISO/IEC 17025, beverages.