Validación del método recuento de coliformes totales en harina de camarón usando placas de Petrifilm

Abstract

Method verification is a fundamental requirement to ensure the reliability of analytical results in laboratories accredited to ISO/IEC 17025. In this context, this study aimed to evaluate the performance of the AOAC 991.14 method using Petrifilm™ plates for the enumeration of total coliforms in shrimp meal, a marine-derived matrix with complex physicochemical characteristics that may affect microbial recovery. The study was conducted under an intralaboratory experimental design, following the guidelines of ISO 16140-3, and assessed key analytical performance characteristics, including inclusivity, exclusivity, accuracy, precision, limits of detection and quantification, and measurement uncertainty. The results showed that the method exhibits suitable analytical performance in the studied matrix, fulfilling the acceptance criteria for quantitative microbiological methods in foods. Additionally, controlled variability and adequate recovery of total coliforms were observed under the specific laboratory conditions. In conclusion, the AOAC 991.14 method with Petrifilm™ plates is suitable and reliable for microbiological control of shrimp meal, contributing to analytical quality assurance and compliance with current regulatory requirements. Keywords: total coliforms, shrimp meal, Petrifilm™, AOAC 991.14, method verification.